Keywords
Abstract
The aim of the study is to evaluate the parameters of acute toxicity of B. subtilis IMV B-7724 lectin after its single administration in mice of two strains.
Balb/c (n = 96) and C57Bl (n = 96) mice were used in the study. Ways of lectin administration: subcutaneous (SC), intraperitoneal (IP), oral (per os). The following doses were used: 125, 250, 375, 500 mg/kg of animal weight (SC and IP); 500 mg/kg (per os). The control mice were injected with physiological NaCl
solution. Based on survival and mortality data, the maximum tolerated (LD0) and absolute lethal (LD100) doses were determined.The median lethal dose(LD50) was calculated using the Kerber and Behrens methods.
The LD50 of B. subtilis IMV B-7724 lectin administered per os or SC to Balb/c and C57Bl mice exceeded the maximum studied dose (500 mg/kg). IP injection of B. subtilis IMV B-7724 lectin was accompanied by a dose-dependent toxic effect: LD0 = 125 mg/kg, LD16 = 132 mg/kg, LD100 = 500 mg/kg. The LD50 values
calculated by two methods did not differ significantly and were equal to 278,8–281,3 mg/kg (for Balb/c mice) and 231,1–250,0 mg/kg (for C57Bl mice).
Evaluation of acute toxicity parameters allows us to classify the studied B. subtilis IMV B-7724 lectin as a low-toxic substance. The results obtained point to the perspectives of carrying out further preclinical studies with the aim of developing a new therapeutic agent based on this bacterial lectin.