Keywords
Abstract
The human need for plant proteins is associated with the importance of amino acids intake. The deficiency of at least one essential amino acid makes a person's diet poor, which can lead to the development of many pathological conditions.
Based on this, one of the topical issues of modern pharmacy is the search for new sources of plant proteins and amino acids that can be used to expand the range of biologically active supplements and drugs.
The aim of the work is to determine the qualitative and quantitative composition of amino acids in the grass and roots of Teucrium сhamaedrys L. and Teucrium montanum L.
To determine the number of amino acids, we used the technique proposed by Stein and Moore in a modern modification, using an automatic amino acid analyzer of the AAA T – 339 model (Czech Republic).
A pre-prepared sample was dissolved in a citrate buffer solution and subjected to analysis. The distribution of the components was carried out on an analytical column, which was filled with an LG ANB cation exchanger. For registration of amino acids, the ninhydrin detection method was used.
The data obtained indicate the presence of 18 amino acids in all samples of the plant materials, 7 of which are essencial.
The largest amount of amino acids was found in the grass of Teucrium сhamaedrys L. – 7.161 mg/100 mg. The amino acid content in the roots of Teucrium сhamaedrys L. and grass of Teucrium montanum L. is almost the same 3.853 mg/100 mg and 3.703 mg/100 mg, respectively. The smallest amount of amino
acids is found in the roots of Teucrium montanum L. – 2,391 mg/ 100 mg.
Aspartic and glutamic acids are present in the largest quantities in samples. Among the predominant amino acids, it is worth noting leucine, glycine, lysine and alanine, the concentration of which in the objects is in the range of 5–10 % of the total amino acid content.
The results of the investigation expand the existing information about the amino acid composition of the grass and roots of Teucrium сhamaedrys L. and Teucrium montanum L.