Keywords
Abstract
The aim was to investigate the effect of a thick extract of Tanacetum parthenium L. (TETP) on the secretory function of the stomach and its acute toxicity. An acute toxicity study of TETP was conducted on 24 white rats (12 males, 12 females) weighing 160–180 g, aged 3.5–4 months. TETP was administered at a dose of 5000 mg/kg once intragastrically in the form of an aqueous suspension stabilized with Tween-80 in a maximum volume of 2 ml/100 g of body weight. Control animals were given an equivalent amount of water. The effect of TETP on experimental animals was studied for 14 days according to their behavior, food consumption and body weight dynamics, and at the end of the experiment the condition of internal organs was assessed: liver¸ kidneys, heart, lungs, spleen, adrenal glands, thymus, testicles by macroscopic examination and organ's mass coefficients. The study of the effect of TETP on the secretory function of the stomach was carried out according to the method of N. I. Andreeva and S. D. Sharova. TETP was administered to animals once intragastrically at a dose of 50 mg/kg after 48 hours of food deprivation, without restriction of water intake. Control animals were given an equivalent amount of water. The results of the study showed that an increase in body weight of animals in all groups had positive dynamics. The condition of the internal organs, which was evaluated macroscopically during the anatomical dissection of all animals, did not differ from that of the negative control animals. The results of the study of the effect on the secretory function of the stomach showed that the administration of TETP at a dose of 50 mg/kg increased to some extent the intensity of gastric juice secretion (by 11 %) and the total acidity of gastric juice (by 5 %), but it was not reliable compared to the control. The fractional composition of the acidity of gastric juice was not disturbed: free and bound acidity fluctuated within the limits of the control group. It was established that TETP when administered intragastrically to both male and female rats is practically non-toxic and according to the classification of K. K. Sydorov belongs to the 5th class of toxicity (LD50 > 5000 mg/kg). TETP at a dose of 50 mg/kg does not affect the intensity of gastric juice secretion and its acidity.