Keywords
Abstract
The aim of the study was to analyze lipophilic extract of the herb Equisetum arvense L. by GC-MS
method to search for marker's substances.
The lipophilic extract of the herb Equisetum arvense L. was analyzed on an Agilent gas chromatograph-
mass spectrometer (GCMS) Model 6890/5973, equipped with a DB-5ms fused silica capillary column containing (5 %-Phenyl)-methylpolysiloxane stationary phase (30 m length x 0.25 mm internal diameter and 0.25 μm film thickness), programmed from 50 °C (5 min) to 320 °C at 4 °C/min and held for 5 min. The identification of components was based on retention indices and computer matching with the WILLEY 275.L library, as well as by comparison of the fragmentation patterns of mass spectra with those reported in the literature. The proportions of the identified compounds were calculated by internal normalization.
Twenty three constituents were determined in the herb Equisetum arvense L. lipophilic extract. The main constituents were found to be palmitic acid (91,33 mg/kg), phytol (44,93 mg/kg), trans-neofitadien (35,07 mg/kg), γ-sitosterol (31,26 mg/kg), hexacozanal (28,36 mg/kg) and linoleic acid (24,01 mg/kg).
As a result, marker's substances were identified among the main constituents of this lipophilic extract for standardization of Equisetum arvense L. in medicinal mixture, such as: γ-sitosterol, phytol, trans- neofitadien and hexacozanal.