Keywords
Abstract
Enhancement of NO metabolism induceds the violations of vascular tone, which are significant manifestation of pathological effect of high doses of radiation on the cardiovascular system. The aim of exploration was to study quercetin influence on NO-dependent blood vessels reactions recovery involving endothelium and perivascular adipose tissue (PVAT) in rats after single sublethal X-irradiation (7 Gy); particularly, to compare contractile and dilatory reactions of thoracic aorta (TA) and mesenteric artery (MA) fragments from control and irradiated animals as well as irradiated animals exposured to quercetin (Quertin, BCPР). The experiments were performed on isolated blood vessels from adult (7–8 months) male rats. Irradiated animals group exposured to quercetin for 14 days (per os, 10 mg/kg). The preliminary contractions of MA and TA rings, with removed PVAT (PVAT–) or left native (PVAT+) were performed in presence of KCl
(60 мМ) and phenylephrine (3 • 10–6 М). The accumulation of NO was evaluated by the rings contractile reactions to NOS inhibitor (L-NAME, 10–5 М) and dilatory reactions to N-acetylcysteine (10–4 М) and acetylcholine (dose-response). Control group blood vessels reactions indicated low level of spontaneous NO production and deposition with the participation of the endothelium (TA) and perivascular adipose tissue (MA). Significant changes in contractile and dilatory blood vessels reactions after X-irradiation related to enhanced level of spontaneous NO production in endothelium (MA) and PVAT (TA) were observed. Course exposition to quercetin in irradiated animals led to decreasing of spontaneous NO production and blood vessels contractile activity normalization. Our data indicates quercetin based medicines to become effective instrumentation in post-radiation cardiovascular disturbances corrections.