Keywords
Abstract
With the stable demand of the pharmaceutical industry for medicinal plant raw materials and the need
to search for new herbal remedies, special attention should be paid to plants that have a widely repre- sented raw material base. The study of such plants is promising from the point of view of the medicinal demand for new effective remedies.
One of such promising plants is Achillea collina J. Becker et Reichenb., the raw material base of which is widely represented in Ukraine. Analysis of sources in scientific literature has shown that recently a sig- nificant amount of research has been devoted to the study of the content and composition of the essential oil, which determines the biological effect of plant materials in officinal A. millefolium. But data on the accumulation during the growing season of essential oil in the promising essential oil species Achillea col- lina J. Becker et Reichenb. are rather limited.
The purpose of the work is to study the composition of the volatile compounds of the herb Achillea col- lina J. Becker et Reichenb. using gas chromatography with a mass spectrometric detection.
The essential oil components were identified by TLC in the mobile phase of ethyl acetate – toluene (5 : 95). Volatile compounds were obtained from the raw material Achillea collina J. Becker et Reichenb. According to the Klevenger method in the equipment in accordance with the requirements of SPU (2.8.12).
The study of volatile compounds was carried out by gas chromatography with mass detection (GC-MS). By this method there were established the presence of 39 compounds in the essential oil of the herb Achillea collina J. Becker et Reichenb., 37 of them identified, which accounted for 94.80 % of the total number of compounds. The dominant components were: chamazulene (28.61 % ± 1.75 %), karyo- philene (11.60 % ± 0.46 %), δ-cadinen (10.28 % ± 0.13 %), terpinene-4-ol (8.81 % ± 0.07 %) and karyo- philene oxide (4.81 % ± 0.07 %). Analysis of the data obtained showed that the dominant components of the essential oil of the studied species are the following monoterpenes: α-pinene, β-pinene, sabinene, 1.8-cineole. Sesquiterpenoids are represented by chamazulene and its derivatives, karyophilene, δ-cadinene, karyophilene oxide.
Thus, the data obtained allow us to recommend the raw material of Achillea collina J. Becker et Reichenb. for drugs development with anti-inflammatory and antibacterial actions.