Keywords
Abstract
A promising direction for improving the procedure for standardization of multicomponent phytoremedies is the use of so-called marker compounds, or markers substances, which are characteristic only for indi- vidual medicinal raw materials. The introduction of methods of qualitative and quantitative analysis based on the use of markers is not only of great practical importance, but also of significant scientific expediency.
The fruits of coriander are among the most commonly components used to make complex herbal col- lections for medical practice both in the form of mono preparation and in the form of components of over- the-counter medicinal products of herbal origin. The pharmacological activity of the coriander fruits is due to the presence in their composition of a complex of biologically active substances, in particular terpe- noids, the content of which is associated with such pharmacological properties of the plant as bactericidal, anti-inflammatory, etc. That is why it was considered expedient to search for markers to standardize this plant in mixtures among terpenoid components.
The aim of the study was to analyze the components of the lipophilic extract of coriander fruits by the method of gas chromatography with mass detection (GC-MS) to search for marker substances for stan- dardization of the plant in mixtures.
The object of the study was coriander fruits in packs of 100 g (manufacturer: Liktravy, Ukraine). The lipophilic extract of the studied raw material was obtained by steam distillation of the raw material. The study of components of the lipophilic extract of coriander fruits was carried out on Agilent 6890 gas chromatograph equipped with a mass spectrometric detector (model 5973). The identification of the studied components was carried out by mass spectra and the retention time of the components.
Twenty two constituents were determined in the Coriander fruits lipophilic extract. The major component of the lipophilic extract is the terpenoid linalool, the content of which among the total amount of vola- tile compounds is 85.812% ± 9.611%.
The following components are characterized by a significantly lower content: camphor (5.021% ± 0.422%), geraniol (5.155% ± 0.624%) and geranyl acetate (1.356% ± 0.142%). The content of other indi- vidual components was less than 1% of the mass of volatile components of the studied extract.
Thus, based on results obtained among the main constituents of lipophilic extract of the Coriander fruits have been identified linalool, camphor, geraniol, and geranyl acetate as markers for standardization of this plant in medicinal mixtures.