Keywords
Abstract
The aim of the study is to research the content of biologically active polyphenolic compounds in the
grass of Achillea collina J. Becker et Reichenb. and assess the possibility of developing new medicines based on it.
A promising representative of the genus Achillea L. In flora of Ukraine is Achillea collina J. Becker et Reichenb., which has a sufficient raw material base in Ukraine. During the growing season from June to October, the grass of Achillea collina J. Becker et Reichenb. accumulates a variety of biologically active substances of broad-ranging chemical composition, among which polyphenolic compounds attract spe- cial attention. They belong to the group of antioxidants and suppress free radical oxidation. Polyphenolic compounds exhibit the properties of scavengers of reactive forms of oxygen and NO, inhibit lipoperoxida- tion of phospholipids in biological membranes of the of target organs, enhance the expression of glutathi- one peroxidase, inhibit the synthesis of pro-inflammatory factors and apoptosis caused by free radicals.
Identification of the polyphenolic compounds was carried out by the method of thin layer chromatogra- phy of ethyl acetate extracts from leaves, inflorescences and grass of the studied plant. To determine the quantitative content of the sum of polyphenolic compounds, the method of UV spectroscopy was used, measuring the optical density of the extracts on the spectrophotometer «Specord-200 UV/Vis Lambda 365 (Analytic Jena)» at wavelength of 390–490 nm in a 10 mm cuvettes with luteolin as a standard sample.
Polyphenolic compounds were found in the extracts of Achillea collina J. Becker et Reichenb. The high- est content of polyphenolic compounds was observed in the extract obtained from the grass of Achillea collina J. Becker et Reichenb. (2.66 mg % ± 0.24 mg %), the lowest content – in extracts from the follia (0,035 mg % ± 0,020 mg %).
Thus, the results of the analysis allow us to consider the grass of Achillea collina J. Becker et Reichenb. as a promising raw material for developing the composition of new herbal remedies of complex action due to the content of biologically active polyphenolic compounds.